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PIXUL Multi-Sample Sonicator: Extremely Consistent High-Throughput Shearing for Multi-Omics Applications

How proteomics labs eliminate the sample-prep bottleneck by sonicating 1 to 96 samples in parallel in standard 96-well plates — with LC-MS/MS results equivalent to probe sonication.

This two-page reference introduces PIXUL — a benchtop ultrasonicator that processes 1 to 96 samples simultaneously in standard cell-culture plates with no proprietary consumables. Page one covers the 3-step workflow (cool, program, sonicate) and five engineered properties: consistent, simple, fast, flexible, and affordable. Page two presents a complete bottom-up proteomics validation against probe sonication on HEK293T cells and mouse liver tissue, with head-to-head LC-MS/MS data on peptide identification, protein groups, and post-translational modification artifacts.

Read or download to see:

  • 1 to 96 samples simultaneously in standard 96-well cell-culture plates — arrayed transducer architecture delivers uniform acoustic energy to every well, with up to 12 different sonication conditions programmable per run.
  • Ready to sonicate in 6 minutes from cold start via single-button startup, with all 96 samples processed in 30 minutes — no degassing, no proprietary tubes, no external computer required.
  • Equivalent LC-MS/MS performance on HEK293T cells — 9,532 peptides and 2,368 protein groups identified by PIXUL versus 9,585 peptides and 2,440 protein groups by probe sonication, with comparable N-terminal acetylation (2.70% vs 2.47%) and methionine oxidation (11.72% vs 11.09%).
  • Optimized 30-minute tissue protocol on mouse liver — PIXUL identifies 8,048 peptides (+1.1%) and 1,573 protein groups (+4.0%) versus probe sonication, validated across a 5/15/30/60-minute time course at 50 N pulse, 1 kHz, 20 Hz burst rate.
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